TRIM47 promotes malignant progression of renal cell carcinoma by degrading P53 through ubiquitination
نویسندگان
چکیده
Abstract Background Renal cell carcinoma (RCC) is one of the most common malignant tumors originating from renal parenchymal urinary epithelial system. Tripartite motif 47 (TRIM47) a member TRIM family proteins, which has E3 ligase activity and been demonstrated to be involved in occurrence prognosis many tumors. The main purpose this study explore role potential mechanism TRIM47 promoting biological behavior RCC. Materials methods mRNA protein levels human cancer paired normal adjacent tissues were detected by qRT-PCR Western blot. effects knockdown overexpression cells on proliferation, invasion xenograft tumor growth nude mice analyzed. molecular was explored mass spectrometric exploration,Western blot immunoprecipitation assays. Results promoted RCC proliferation vitro vivo as an oncogene. Mechanistically, exerted interacting with P53 increase its ubiquitination degradation, further Conclusions Our that TRIM47-P53 axis played functional progression suggested therapeutic target for
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ژورنال
عنوان ژورنال: Cancer Cell International
سال: 2021
ISSN: ['1475-2867']
DOI: https://doi.org/10.1186/s12935-021-01831-0